- Title
- Founder mutations for early onset melanoma as revealed by whole exome sequencing suggests that this is not associated with the increasing incidence of melanoma in Poland
- Creator
- Debniak, Tadeusz; Scott, Rodney J.; Kashyap, Amiruddh; Lener, Marcin R.; Malinska, Karolina; Rogoza, Emilia; Murawa, David; Rudnicka, Helena; Deptula, Jakub; Lubinski, Jan; Lea, Rodney A.; Górski, Bohdan; Masojc, Bartlomiej; Cybulski, Cezary; Kram, Andrzej; Maleszka, Romuald; Gromowski, Tomasz; Paszkowska-Szczur, Katarzyna
- Relation
- Cancer Research and Treatment Vol. 51, Issue 1, p. 337-344
- Publisher Link
- http://dx.doi.org/10.4143/crt.2018.157
- Publisher
- Korean Cancer Association
- Resource Type
- journal article
- Date
- 2019
- Description
- Purpose: Germline mutations within melanoma susceptibility genes are present only in minority of melanoma patients and it is expected that additional genes will be discovered with next generation sequence technology and whole-exome sequencing (WES). Materials and Methods: Herein we performed WES on a cohort of 96 unrelated Polish patients with melanoma diagnosed under the age of 40 years who all screened negative for the presence of CDKN2A-variants. A replication study using a set of 1,200 melanoma patient DNA samples and similarly large series of healthy controls was undertaken. Results: We selected 21 potentially deleterious variants in 20 genes (VRK1, MYCT1, DNAH14, CASC3, MS4A12, PRC1, WWOX, CARD6, EXO5, CASC3, CASP8AP2, STK33, SAMD11, CNDP2, CPNE1, EFCAB6, CABLES1, LEKR1, NUDT17, and RRP15), which were identified by WES and confirmed by Sanger sequencing for an association study. Evaluation of the allele distribution among carriers and their relatives in available family trios revealed that these variants were unlikely to account for many familial cases of melanoma. Replication study revealed no statistically significant differences between cases and controls. Conclusion: Although most of the changes seemed to be neutral we could not exclude an association between variants in VRK1, CREB3L3, EXO5, and STK33 with melanoma risk.
- Subject
- whole exome sequencing; melanoma; frameshift mutation; SDG 3; Sustainable Development Goals
- Identifier
- http://hdl.handle.net/1959.13/1454687
- Identifier
- uon:44984
- Identifier
- ISSN:1598-2998
- Rights
- Copyright © 2019 by the Korean Cancer Association. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
- Language
- eng
- Full Text
- Reviewed
- Hits: 5247
- Visitors: 5299
- Downloads: 201
Collections
Goal 3: Good Health and Well-Being
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | ATTACHMENT02 | Publisher version (open access) | 593 KB | Adobe Acrobat PDF | View Details Download |